By John E. Hall (auth.), Donna H. Wang (eds.)
The renin-angiotensin method has been within the highlight for greater than a hundred years due to its important position in blood strain legislation, ion and fluid homeostasis, and tissue development and transforming. contemporary advances in molecular genetic recommendations have tested that the renin-angiotensin approach may possibly carry the major to the pathogenesis of high blood pressure, congestive middle failure, power renal failure, and stroke. In Angiotensin Protocols, Donna Wang and a panel of hands-on specialists describe in step by step aspect all of the significant molecular, biochemical, and useful recommendations now utilized in probing this strong method. those variety from using embryonic gene concentrating on to create mutant traces, to the sensible research of elements of the renin-angiotensin procedure. Following the winning trend of the equipment in Molecular Biology™ sequence, each one procedure is crafted for experimental luck with certain directions, perceptive notes highlighting the main serious steps, and priceless tips about pitfalls to avoid.
complete and hugely useful, Angiotensin Protocols interprets professional wisdom into comfortably reproducible tools that let all biomedical investigators effectively to discover the function of angiotensin in overall healthiness and affliction this present day.
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A. (1973) Intrarenal role of angiotensin II. homeostatic regulation of renal blood flow. Circ. Res. 32, 692–698. 44. Hall, J. , Guyton, A. , Trippodo, N. , Lohmeier, T. , McCaa, R. , and Cowley, A. , Jr. (1977) Intrarenal control of electrolyte excretion by angiotensin II. Am. J. Physiol. 232, F538–F544. 45. Kimbrough, H. , Vaughn, E. , Carey, R. , and Ayers, C. R. (1977) Effect of intrarenal angiotensin II blockade on renal function in conscious dogs. Circ. Res. 40, 174–178. 46. Hall, J. , Guyton, A.
Blood Pressure 7, 71–75. 2. Page, I. H. (1987) Hypertension Mechanisms. Grune & Stratton, Orlando, FL. 3. Robertson, J. I. S. (1993) Renin and angiotensin: an historical review, in The Renin–Angiotensin System (Robertson, J. I. S. and Nicholls, M. ), Gower, London, pp. 1–18. 4. Aurell, M. (1998) Robert Tigerstedt—scientist, teacher and critic, in Renin– Angiotensin (Ulfendahl, H. R. ), Portland Press, London, pp. 1–12. 5. Skeggs, L. (1986) Current concepts and historical perspectives of renal pressor mechanisms.
Mann, J. and Julius S. (1998) The Valsartan Antihypertensive Long-term Use Evaluation (VALUE) trial of cardiovascular events in hypertension. Rationale and design. Blood Pressure 7, 176–183. Production and Use of Chimeric Mice II TRANSGENIC AND GENE TARGETING METHODS 39 Production and Use of Chimeric Mice 41 3 Production and Use of Chimeric Mice Junji Takaya, Taiji Matsusaka, and Iekuni Ichikawa 1. Introduction The gene-targeting technology allows complete and selective inactivation of a specific gene to study the consequence of total absence of the gene product.